Volume-sensitive chloride conductance in bovine chromaffin cell membrane.

Extracellular adenosine modulates a volume-sensitive-like chloride conductance in immortalized rabbit DC1 cells.

Cl(-) currents induced by cell swelling were characterized in an immortalized cell line (DC1) derived from rabbit distal bright convoluted tubule by the whole cell patch-clamp techniques and by (125)I(-) efflux experiments. Exposure of cells to a hypotonic shock induced outwardly rectifying Cl(-) currents that could be blocked by 0.1 mM 5-nitro-2-(3-phenylpropyl-amino)benzoic acid, 1 mM DIDS, a...

Regulation of cell proliferation by intermediate-conductance Ca -activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells

Tao R, Lau CP, Tse HF, Li GR. Regulation of cell proliferation by intermediate-conductance Ca -activated potassium and volumesensitive chloride channels in mouse mesenchymal stem cells. Am J Physiol Cell Physiol 295: C1409–C1416, 2008. First published September 24, 2008; doi:10.1152/ajpcell.00268.2008.—Bone marrow mesenchymal stem cells (MSCs) are a promising cell source for regenerative medici...

Regulation of cell proliferation by intermediate-conductance Ca2+-activated potassium and volume-sensitive chloride channels in mouse mesenchymal stem cells.

Bone marrow mesenchymal stem cells (MSCs) are a promising cell source for regenerative medicine; however, their cellular physiology is not fully understood. The present study aimed at exploring the potential roles of the two dominant functional ion channels, intermediate-conductance Ca(2+)-activated potassium (IK(Ca)) and volume-sensitive chloride (I(Cl.vol)) channels, in regulating proliferati...

Volume-Sensitive Chloride Channels Do Not

Hydroxytryptamine transport by the bovine chromaffin-granule membrane.

5-Hydroxytryptamine is accumulated by resealed chromaffin-granule 'ghosts' if a pH gradient (acid inside) is imposed across their membranes by preincubating them at low pH. This uptake, like that driven by MgATP, is reserpine- and uncoupler-sensitive. This strongly suggests that catecholamines are taken up by intact granules in response to a pH gradient. In line with this, it is shown that 5-hy...